In vitro susceptibility of rainbow trout fin cells to viral haemorrhagic septicaemia virus
نویسندگان
چکیده
Cells isolated from rainbow trout fins Oncorhynchus mykiss, Walbaum were propagated and subcultured a s fibroblast-like. Viral haemorrhagic septicaemia virus (VHSV) replicated in the cultured fin cells as demonstrated by fin cell lysis, posihve immunofluorescence with polyclonal antiVHSV, and Increase in titre as measured by either infection of f ~ s h cell lines or by sandwich ELISA with anti-VHSV monoclonal antibodies. The possible use of these fln cell cultures as VHSV-antigen presenting cells for immunological studies is briefly discussed.
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Spectrophotometric method for titration of trout interferon, and its application to rainbow trout fry experimentally infected with viral haemorrhagic septicaemia virus
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